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Gene expression analysis using the Broad Institute Connectivity Map The authors analysed how GHK-Cu affects human gene expression using the Connectivity Map (CMap) a massive database developed at MIT and Harvard that maps how compounds alter gene activity

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Immunohistochemistry test Rat abdominal subcutaneous adipose tissue slices were immersed in antigen retrieval solution (P0086, Beyotime, Shanghai, China) and boiled at 95C for 10 min to repair antigen

Hair follicles undergo continuous cycles of growth (anagen), regression (catagen), and rest (telogen) phases, with the duration and quality of anagen determining overall hair production and appearance